Some journal articles may be available online from: Ingenta or Science Direct
R5902Cb Development of diagnostic systems for the study of epidemeology and taxonomy of the Aphanomyces fungus associated with tropical freshwater fisheries.
Other Output
Hart, D. (1997). Development of diagnostic systems for the study of the molecular epidememiology and taxonomy of the Aphnomyces fungus associated with EUS of tropical and freshwater fishes. Final report of the DFID Research Project R5902Cb. 1st October 1993 to 31st March 1997.
R5680 Consultancy in tropical mycology
Other Outputs
Willoughby L.G. (1994). Consultancy in tropical mycology. Final Technical Report. pp 7 [view pdf file: 0.5MB]
Willoughby L.G. (1994). Handbook of Tropical Fish Mycology. Pisces Press, Stirling 72p.
Willoughby L.G. (1994). Aphanomyces invaderis the fungal pathogen of EUS. C/N ratios and Morphogenesis. AAHRI Newsletter, May 1994, pp2-3.
Willoughby L.G. & Roberts R.J. (1994). Improved methodology for isolation of the Aphanomyces fungal pathogen of Epizootic Ulcerative Syndrome (EUS) of Asian fish. J. Fish Dis. 17, pp. 541-544.
Chinabut S., Roberts R.J., Willoughby L.G. & Pearson M.D. (1995). Histopathology of snakehead Channa striata (Bloch) experimentally infected.
R5525 Development of tropical crustacean cell cultures
Peer Reviewed
Frerichs, G.N. (1996). In vitro culture of embryonic cells from freshwater prawn,
Macrobrachium rosenbergii. Aquaculture 143, 227-232.
Non Peer
Frerichs, G.N. and Tweedie, A. (1995). In vitro cultivation of crustacean cells. Aquaculture
News 20, 27-28.
Other Outputs
Frerichs, G.N. (1996). Development of tropical crustacean cell cultures. Final Project Report. pp 05 [view pdf file: 0.5MB]
R5502 Development of transgenic fish
Peer Reviewed
Alam, S. Popplewell A and MacLean N. (1996). Germline transmission and expression of a lacZ containing transgene in tilapia Oreochromis niloticus. Transgenic Research 5, 87-95.
Alam, M.S., Lavender, F.L, Iyengar A., Rahman M.A., Ayad, H.H, Lathe, R., Morlay, S.D and
MacLean, N. (1996). Comparison of the activity of carp and rat B-actin gene regulatory sequence in tilapia and rainbow trout embryos. Molec. Reprod. Develop. 45, 117-122.
Bardakci, F. and Skibinski, D.O.F. (1994). Application of the RAPD technique in tilapia fish: species and subspecies identification. Heredity 73, 117-123.
Brookfield, J.F.Y., Carter, R.E., Mair, G.C. and Skibinski, D.O.F. (1993). A case study of the interpretation of the linkage data using DNA fingertipping probes. Molecular Ecology 2, 209-218.
Hanley, S, Muller, F., MacLean, N. and Smith T. (1996). Isolation and functional analysis of the histone H3 promoter from Atlantic salmon (Salmo salar). Abstracts of EC Biotechnology Programme Symposium Norwich UK.
Iyengar, A and MacLean, N. (1995). Transgene concatamerization and expression in Rainbow trout. Molec Marine Biology and Biotechnology 4, 248-254.
Iyengar, A., Muller, F, and MacLean, N. (1996). Expression and regulation of transgenes in fish. Transgenic Research 5., 147-165.
MacLean, N. (1995). Animals with Novel Genes. 266pp (Ed.) Cambridge University Press.
MacLean, N, and Rahman, A. (1995). Transgenic Fish. In: Animals with Novel Genes. (Ed. N. MacLean). pp 63-105. Cambridge University Press.
MacLean, N., Alam, S., Iyengar, A and Popplewell, A. (1996). Transient expression of reporter genes in fish as a measure of promoter efficiency. pp. 165-174. In: Gene expression and Manipulation in aquatic Organisms (Eds. Goldspink and Ennion) SEB Seminar Series. Cambridge University Press.
Muller, F., Gauvry, L., Williams, D.W., Kobolak, J., MacLean, N., Orban, L. and Goldspink, G. (1996). The use of transient lacZ expression in fish embryos for comparative analysis of cloned regulatory elements. pp 175-199. In: Gene expression and Manipulation in aquatic Organisms. (Eds. Goldspink and Ennion) SEB Seminar Series . Cambridge University Press.
Muller, F, F., Williams, D.W., Kobolak, J., Orban, L, Goldspink, G and MacLean N. (1996). Tissue specific activator effect of enhancers on the expression of minimal promoters in coinjected zebrafish embryos provides a rapid enhancer assay. In: Proc Zebrafish Development and Genetics Symposium, Cold Spring Harbour Laboratory, New York.
Muller, F., Williams, D.W., Kobolak, J., Gauvry, L., Goldspink., Orban, L and MacLean, N. (1997). Activator effect of coinjected enhancers on the muscle specific expression of a myosin heavy chain promoter in zebrafish embryo. Molec. Reprod. Develop. 47, 404-412.
Naish, K.A, Warren M., Bardakci, Skibinski, D.O.F., Carvalho, G.R. and Mair, G.C (1995). Multi locus DNA fingerprinting and RAPD reveal similar genetic relationships between strains of Oreochromis niloticus (Pices:Cichlidae). Molecular Ecology 4, 271-274.
Rahman, M.A. and MacLean N. (1997). Coinjection strategy improves integration efficiency and germline transmission of a growth hormone gene construct in tilapia (Orechromis niloticus). Transgenics research 6, 369-378.
MacLean and Rahman M.A. (1998). Threefold growth enhancement in genetically modified strains of tilapia fish. Proc 6th World Congress on Genetics Applied to Livestock Production Armidale, Australia 27,100-103.
Smith, T., Alestrom, P., Breton, B, Prunet, P., Jego, P., Bailhache T., MacLean N. and Martial J. (1996). Biological containment of transgenic fish and risk assessment of interspecies gene transfer. Abstracts of EC Biotechnology Programme Symposium Norwich UK.
Williams D.W., Muller, F., Lavender, F.L, Orban L. and MacLean N, (1996). The formation and fate of the yolk syncytial layer (YSL) of Danio rerio: A topographic map of YSL nuclei throughout development. Proc Zebrafish Development and Genetics Symposium, Cold Spring Harbour Laboratory, New York
Hanley, S, Muller F., Uzebekova, S, MacLean, N, Prunet P., Breton, B., and Smith T.J. (1998). Isolation and functional analysis of the Histone H3 promoter from the Atlantic salmon (Salmo salar). Molec,. Marine Biol. & Biotech.
MacLean, N. (1998). Regualtion and exploitation of transgenes in fish Mutation Res.
MacLean N. "Genetic manipulation of Farmed Fish". In: The Biology of Farmed Fish: Ed. K. Black, Sheffield Academic Press.
MacLean, N. Regualtion and exploitation of transgenes in fish. Mutation Research.
Naish , K.A and Skibinski D.O.F. Tetranucleotide microsatellite loci for Indian carp Catla catla, J. Fish Biology.
Rahman, M.A., Mak, R., Ayad, H., Smith, A and MacLean N. (1998). Expression of a novel piscine growth hormone gene results in growth enhancement in transgenic tilapia. Transgenic Research 7, 357-370.
Rahman, M.A. and MacLean N. (1999). Observation of growth performance of transgenic tilapia containing an exogenous piscine growth hormone gene. Aquaculture 173, 333-346
Rahman, M.A and MacLean, N. (1998). Production of lines of growth enhanced transgenic tilapia expressing a novel piscine growth hormone gene. In: Proceedings of 4th International Marine Biotechnology Conference, Sorrento Italy.
Rahman, M.A and MacLean, N. (1997). Growth enhanced transgenic tilapia (Oreochromis niloticus). In: "Tilapia Aquaculture"-Proceedings of 4th International Symposium on Tilapia in Aquaculture, Orlando, Florida, 1,97-108.
Other Outputs
Naish, K.A, Leamon, J.P and Skibinski, D.O.F. Addition of expressed sequence tags to linkage groups in tilapia using universal PCR primers designed from fish cDNA sequences. (Unpublished manuscript)
R5430 Studies on viruses associated with ulcerative disease of fish in the Indo-Pacific region
Peer Reviewed
Frerichs, G.N. (1995). Viruses associated with the Epizootic Ulcerative Syndrome (EUS) of fish in South-East Asia. Veterinary
Research 26, 449-454.
Frerichs, G.N., Rodgers, H.D. and Peric, Z. (1996). Cell culture isolation of piscine neuropathy nodavirus (PNN) from juvenile sea bass,
Dicentrachus labrax. Journal of General Virology 77, 2067-2071.
Frerichs, G.N. (1996). Identification and elimination of mycoplasmas in fish cell line cultures. Journal of Fish Diseases, 19: 435-439.
Hart, D. and Frerichs, G.N. (1995). Retroviruses from South-East Asian fish. Aquaculture News,
19, 24-25
Hart, D., Frerichs, G.N., Rambaut, A. and Onions, D.E. (1996). Complete nucleotide sequence and transcriptional analysis of the snakehead fish retrovirus. Journal of Virology, 70
(6), 3606-3616.
Karunasager, I., Millar, S., and Freichs, G.N. (1995). A new cell line from Puntius schwanenfeldi sensitive to snakehead fish cell C-line retrovirus. Asian Fisheries Science 8, 151-157
Lilley, J.H. and Frerichs, G.N. (1994). Comparative analysis of structural, serological and cytopathogic properties of rhabdovirus associated with Epizootic Ulcerative Syndrome (EUS). Journal of Fish
Disease 17 (5), 522.
Non Peer
Kanchanakhan, S. (1996). Epizootic ulcerative syndrome (EUS) a new look at the old story. The AAHRI Newsletter, 5(1), Pp. 2-3. Aquatic Animal Health Research Institute, Bangkok.
Kanchanakhan, S. (1997). Varability in the isoloation of viruses from fish affected by epizootic ulcerative syndrome (EUS). The AAHRI Newsletter, 6(1), pp 2-3. Aquatic Animal
Health Research Institute, Bangkok.
Other Outputs
Rodger, G.N. (1997) Studies on virus infections of food fish in the Indo-Pacific region. Final Project Report. pp 15 [view pdf file: 0.03MB]
R5069 Development of Transgenic Tilapia Fish
Peer Reviewed
MacLean, N, Iyengar, A and Rahman, A. (1993). Transgene expression and transmission in rainbow trout (Onchorynchus mykiss) and tilapia (Oreochromis niloticus) In: Symposium of Br. Cell Biology and Br. Soc. Development Biology, Brighton, 1993.
MacLean N. (1993). Transgenic Induction in Salmonid and Tilapia fish. In: Murphy, D and Carter, D.A (Eds.) Transgenic techniques Principles and Protocols, Humana Press, New Jersey, USA, 95-108.
Non Peer
Iyengar, A and MacLean, N. (1993). Fish and mammalian promoters in transgenic fish, In: Proceedings of 17th International Congress of Genetics: Workshop on Transgenic Fish, Birmingham,
MacLean, N. (1993). Report on transgenic fish workshop, Birmingham. Ag . Biotech News
MacLean, N. (1993). Progress with transgenic fish. Proceedings of Symposium on Transgenic Animals, Swedish Society for Developmental Biology Stockholm, 1993.
MacLean, N. (1993). Optimising transgene expression in fish. In: Report of the 5th ELWW Meeting on Fish Genes and the Regulation of their Expression, University College, Galway.
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